产品货号：
GS4465
中文名称：
重组人IGF-BP3
英文名称：
Recombinant Human Insulin-like Growth Factor-Binding Protein 3
产品规格：
5μg
发货周期：
1～3天
产品价格：
询价
分子量：Approximately 28.8kDa,a single non-glycosylated polypeptide chain containing 264 amino acids.
储存条件：冷冻（-20℃）
概述
Insulin-like Growth Factor-Binding Protein 3(IGF-BP3)belongs to the IGFBP family,which are all cysteinerich proteins with conserved cysteine and have an IGFBP domain and a thyroglobulin type-I domain.Mature human IGF-BP3 contains 264 a.a.with three potential N-linked and two potential O-linked glycosylation sites.It is expressed by most tissues and has higher levels during extrauterine life and peak during puberty.The expression of IGF-BP3 in fibroblasts is stimulated by mitogenic growth factors such as Bombesin,Vasopressin,PDGF,and EGF.The protein forms a ternary complex with IGF-I or II and acid-labile subunit.In this form,it circulates in the plasma,prolonging the half-life of IGFs and altering their interaction with cell surface receptors.Decreased plasma levels of IGF-BP3 often happen during the progression of prostate cancer from benign to metastatic disease.
使用说明
This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
技术规格
相关搜索：重组人IGF-BP3，IGF-BP3, Human
储存条件：冷冻（-20℃）
概述
Insulin-like Growth Factor-Binding Protein 3(IGF-BP3)belongs to the IGFBP family,which are all cysteinerich proteins with conserved cysteine and have an IGFBP domain and a thyroglobulin type-I domain.Mature human IGF-BP3 contains 264 a.a.with three potential N-linked and two potential O-linked glycosylation sites.It is expressed by most tissues and has higher levels during extrauterine life and peak during puberty.The expression of IGF-BP3 in fibroblasts is stimulated by mitogenic growth factors such as Bombesin,Vasopressin,PDGF,and EGF.The protein forms a ternary complex with IGF-I or II and acid-labile subunit.In this form,it circulates in the plasma,prolonging the half-life of IGFs and altering their interaction with cell surface receptors.Decreased plasma levels of IGF-BP3 often happen during the progression of prostate cancer from benign to metastatic disease.
使用说明
This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
技术规格
| 外观 | Sterile Filtered White lyophilized(freeze-dried)powder. |
| 纯度 | ＞98%by SDS-PAGE and HPLC analyses. |
| 来源 | Escherichia coli. |
| 生物活性 | Fully biologically active when compared to standard.The ED50 as determined by inhibiting IGF-II induced proliferation of serum free human MCF-7 cells is less than 200ng/ml,corresponding to a specific activity of>5.0×103 IU/mg in the presence of 15 ng/ml of rHuIGF-II. |
| 成分 | Lyophilized from a 0.2μmfiltered concentrated solution in PBS,pH 7.4. |
| 内毒素 | Less than 1 EU/μg of rHuIGF-BP3 as determined by LAL method. |
| 复溶 | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/mL.Stock solutions should be apportioned into working aliquots and stored at ≤ -20 ℃.Further dilutions should be made in appropriate buffered solutions. |
| 氨基酸序列 | GASSGGLGPV VRCEPCDARA LAQCAPPPAV CAELVREPGC GCCLTCALSE GQPCGIYTER CGSGLRCQPS PDEARPLQAL LDGRGLCVNA SAVSRLRAYL LPAPPAPGNA SESEEDRSAG EVESPSVSST HRVSDPKFHP LHSKIIIIKK GHAKDSQRYK VDYESQSTDT QNFSSESKRE TEYGPCRREM EDTLNHLKFL NVLSPRGVHI PNCDKKGFYK KKQCRPSKGR KR |
相关搜索：重组人IGF-BP3，IGF-BP3, Human